Skip to content
Open
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
6 changes: 3 additions & 3 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -3,7 +3,7 @@
- **pandas**
- **numpy**
- **getopt**
- **hmmlearn** (version: 0.2.5)
- **hmmlearn** (required version: 0.2.5)
- **pyarrow**
- **pytables**
- **pickle**
Expand Down Expand Up @@ -54,7 +54,7 @@
- **Optional Parameters:**
- `-l` Minimum footprints allowed per read (default: 0).
- `-r` Enable circular mode (default: off).
- `-b` Column number (0-based) in BED files with reference methylation starts (e.g., 12 for m6A output from fibertools, 28 by default for full output).
- `-b` Column number (0-based) in BED files with reference methylation starts (e.g., 11 for m6A output from fibertools, 28 by default for full output).
- `-s` Chunk size (default: 50000).
- `-e` How many bases to mask on both ends of the read as 0% methylation probability (default is 10). Required due to the fact that fibertools needs a window to call methylations.
- `-m` Minimum fraction of methylations required in a read (default: 0).
Expand All @@ -72,7 +72,7 @@ The repository includes a folder `Example files` containing necessary files to r
### Input Files
- **accessible_probs.tsv** and **inaccessible_probs.tsv** – probability files for accessible and inaccessible regions (`-p` parameter).
- **dm6.fa** – *Drosophila melanogaster* (dm6) reference genome in FASTA format. This file is too large for github, so please download the file yourself.
- **dm6_example.bed** – example FiberHMM m6A-only output file for m6A modifications. You should specify `-b 12` to use the correct column.
- **dm6_example.bed** – example FiberHMM m6A-only output file for m6A modifications. You should specify `-b 11` to use the correct column.

### Expected Output Files
- **dm6_example_model.pickle** – trained model for the example dataset.
Expand Down