Thanks for the great tool! Not sure if this is appropriate here or in Metaeuk repo so apologies.
For SRR36395071, I have a de novo assembly:
Candidozyma+auris.fna.gz
I'm running BUSCO with metaeuk which calls:
metaeuk easy-predict Candidozyma+auris.fna metaeuk/tmp/refseq_db.faa metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/initial_results/Candidozyma+auris.fna metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp --max-intron 130000 --max-seq-len 160000 --min-exon-aa 15 --max-overlap 15 --min-intron 5 --overlap 1 -s 4.5
This unfortunately exits with
Create directory metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/tmp_predict
predictexons metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/contigs metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/targets metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/MetaEuk_calls metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/tmp_predict --sub-mat 'aa:blosum62.out,nucl:nucleotide.out' -a 0 --alignment-mode 2 --alignment-output-mode 0 --wrapped-scoring 0 -e 100 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --alt-ali 0 -c 0 --cov-mode 0 --max-seq-len 160000 --comp-bias-corr 1 --comp-bias-corr-scale 1 --max-rejected 2147483647 --max-accept 2147483647 --add-self-matches 0 --db-load-mode 0 --pca substitution:1.100,context:1.400 --pcb substitution:4.100,context:5.800 --score-bias 0 --realign 0 --realign-score-bias -0.2 --realign-max-seqs 2147483647 --corr-score-weight 0 --gap-open aa:11,nucl:5 --gap-extend aa:1,nucl:2 --zdrop 40 --threads 8 --compressed 0 -v 3 --seed-sub-mat 'aa:VTML80.out,nucl:nucleotide.out' -s 4.5 -k 0 --target-search-mode 0 --k-score seq:2147483647,prof:2147483647 --alph-size aa:21,nucl:5 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 0 --diag-score 1 --exact-kmer-matching 0 --mask 1 --mask-prob 0.9 --mask-lower-case 0 --min-ungapped-score 15 --spaced-kmer-mode 1 --rescore-mode 0 --filter-hits 0 --sort-results 0 --mask-profile 1 --e-profile 0.001 --wg 0 --allow-deletion 0 --filter-msa 1 --filter-min-enable 0 --max-seq-id 0.9 --qid '0.0' --qsc -20 --cov 0 --diff 1000 --pseudo-cnt-mode 0 --min-length 15 --max-length 32734 --max-gaps 2147483647 --contig-start-mode 2 --contig-end-mode 2 --orf-start-mode 1 --forward-frames 1,2,3 --reverse-frames 1,2,3 --translation-table 1 --translate 0 --use-all-table-starts 0 --id-offset 0 --create-lookup 0 --sequence-overlap 0 --sequence-split-mode 1 --headers-split-mode 0 --chain-alignments 0 --merge-query 1 --search-type 0 --start-sens 4 --sens-steps 1 --prefilter-mode 0 --exhaustive-search 0 --exhaustive-search-filter 0 --strand 1 --lca-search 0 --disk-space-limit 0 --force-reuse 0 --remove-tmp-files 0 --metaeuk-eval 0.001 --metaeuk-tcov 0.5 --max-intron 130000 --min-intron 5 --min-exon-aa 15 --max-overlap 15 --max-exon-sets 1 --set-gap-open -1 --set-gap-extend -1 --reverse-fragments 0
extractorfs /home/test/metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/contigs /home/test/metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/tmp_predict/10019616237998200098/nucl_6f --min-length 15 --max-length 32734 --max-gaps 2147483647 --contig-start-mode 2 --contig-end-mode 2 --orf-start-mode 1 --forward-frames 1,2,3 --reverse-frames 1,2,3 --translation-table 1 --translate 0 --use-all-table-starts 0 --id-offset 0 --create-lookup 0 --threads 8 --compressed 0 -v 3
[=================================================================] 100.00% 163 0s 104ms
Time for merging to nucl_6f_h: 0h 0m 0s 61ms
Time for merging to nucl_6f: 0h 0m 0s 90ms
Time for processing: 0h 0m 0s 354ms
translatenucs /home/test/metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/tmp_predict/10019616237998200098/nucl_6f /home/test/metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp/712558938822520512/tmp_predict/10019616237998200098/aa_6f --translation-table 1 --add-orf-stop 0 -v 3 --compressed 0 --threads 8
[=================================================================] 100.00% 472.54K 0s 90ms
Segmentation fault (core dumped)
Error: translatenucs step died
Error: predictexons step died
Running MMSeqs 18-8cc5c and Metaeuk 7.bba0d80 installed in micromamba docker.
Thanks for your consideration!
Thanks for the great tool! Not sure if this is appropriate here or in Metaeuk repo so apologies.
For SRR36395071, I have a de novo assembly:
Candidozyma+auris.fna.gz
I'm running BUSCO with metaeuk which calls:
metaeuk easy-predict Candidozyma+auris.fna metaeuk/tmp/refseq_db.faa metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/initial_results/Candidozyma+auris.fna metaeuk/auto_lineage/run_eukaryota_odb12/metaeuk_output/tmp --max-intron 130000 --max-seq-len 160000 --min-exon-aa 15 --max-overlap 15 --min-intron 5 --overlap 1 -s 4.5This unfortunately exits with
Running MMSeqs 18-8cc5c and Metaeuk 7.bba0d80 installed in micromamba docker.
Thanks for your consideration!