Update README.md

Author: LucianoAD

README.md

@@ -1 +1,100 @@
-You can view the web guide from this link: https://lucianoad.github.io/transcriptomic_data_analysis/
+<h1 align="center">Practical Transcriptomics with R</h1>
+
+<p align="center">
+Educational workflow for transcriptomic preprocessing, GEO data exploration and reproducible bioinformatics analysis
+</p>
+
+<p align="center">
+
+![Status](https://img.shields.io/badge/STATUS-IN%20DEVELOPMENT-orange)
+![License](https://img.shields.io/badge/LICENSE-MIT-green)
+![R](https://img.shields.io/badge/R-Bioinformatics-blue)
+![Quarto](https://img.shields.io/badge/Quarto-Documentation-purple)
+
+</p>
+
+---
+
+## 🌐 Live Project
+
+**Documentation website**
+
+:contentReference[oaicite:0]{index=0}
+
+---
+
+## 📌 Project Summary
+
+Tutorial-style transcriptomics workflow designed to transform raw GEO datasets into analysis-ready data.
+
+The project demonstrates practical skills in:
+
+- GEO data retrieval
+- Metadata filtering
+- Transcriptomic preprocessing
+- Quality control
+- Normalization strategies
+- Reproducible workflows
+- Data preparation for downstream analyses
+
+---
+
+## 🛠 Tech Stack
+
+**Languages & Tools**
+
+`R` `Quarto` `Bioconductor`
+
+**Packages**
+
+`GEOquery` `tidyverse` `limma` `edgeR` `sva` `ggplot2`
+
+---
+
+## 🔬 Workflow
+
+```text
+GEO datasets
+      ↓
+Metadata exploration
+      ↓
+Sample filtering
+      ↓
+Quality control
+      ↓
+Normalization
+      ↓
+Preprocessed dataset
+```
+
+---
+
+## 📂 Repository Structure
+
+```plaintext
+.
+├── Searching_data/
+├── Preprocessing_data/
+├── index.qmd
+├── custom.css
+└── README.md
+```
+
+---
+
+## 🚧 Current Status
+
+Completed:
+
+✔ GEO retrieval  
+✔ Metadata exploration  
+✔ Sample filtering  
+✔ QC visualization  
+✔ Normalization concepts  
+
+Planned:
+
+⬜ Differential expression  
+⬜ Batch correction  
+⬜ RNA-seq workflows  
+⬜ ML applications