Hello,
I regularly use the Labcyte Echo robot for qPCR analyses with 384-well plates. My design includes many different DNA samples. My question concerns the first step of the generate_blank_design_table.py script and the number of reagents to indicate. If I want to analyse 10 DNA samples from the same condition (biological replicates) with the same mix (1 gene), how many reagents should I specify? 11, one by biological samples and one for the gene mix ? In this case, filling in the design_plate file takes a very long time... I've tried to fill in my condition as the reagent and indicated the 10 positions of my samples one after the other, but it doesn't work because only the first position is taken into account. Is there a suitable solution for this type of analysis?
design_table_384-well-plate.xlsx
Hello,
I regularly use the Labcyte Echo robot for qPCR analyses with 384-well plates. My design includes many different DNA samples. My question concerns the first step of the generate_blank_design_table.py script and the number of reagents to indicate. If I want to analyse 10 DNA samples from the same condition (biological replicates) with the same mix (1 gene), how many reagents should I specify? 11, one by biological samples and one for the gene mix ? In this case, filling in the design_plate file takes a very long time... I've tried to fill in my condition as the reagent and indicated the 10 positions of my samples one after the other, but it doesn't work because only the first position is taken into account. Is there a suitable solution for this type of analysis?
design_table_384-well-plate.xlsx